Reza Salahi Moghaddam; Mohammad Hossein Shahir
Volume 23, Issue 4 , January 2022, , Pages 535-548
Abstract
In order to evaluate the response of broiler chicks to different levels of dietary ideal protein (Ross 308 strain recommendation and 10% less) and digestible valine to lysine ratio (71, 74, 77, 80, and 83%) in the starter period (one to 10 days of age), a study was performed using 500-day-old male broilers ...
Read More
In order to evaluate the response of broiler chicks to different levels of dietary ideal protein (Ross 308 strain recommendation and 10% less) and digestible valine to lysine ratio (71, 74, 77, 80, and 83%) in the starter period (one to 10 days of age), a study was performed using 500-day-old male broilers in a 2×5 factorial arrangements based on a completely randomized design including 10 treatments and 5 replications. The results showed that reducing dietary ideal protein level increased body weight, feed intake and improved feed conversion ratio (P<0.01). The percentage of bone ash and calcium content also increased significantly with decreasing dietary ideal protein (P <0.05). The reduction of the dietary ideal protein level decreased the concentrations of serum cholesterol, glucose, total protein, albumin, globulin, calcium, and phosphorus (P<0.05). The highest average body weight at 10 day of age, weight gain, carcass and breast percentages and the lowest feed conversion ratio were observed at 74% of digestible valine to lysine ratio (P<0.05); while the highest average feed intake, thigh percentage, bone ash and calcium percentage were obtained at 77% of digestible valine to lysine ratio (P<0.05). No significant interaction was observed between the two experimental factors except for the carcass percentage traits (P<0.05) and serum phosphorus concentration (P<0.01). In conclusion, based on the results of this study, a 10% reduction in dietary ideal protein levels is possible by maintaining ideal ratios of essential amino acids. The proper ratio of digestible valine to lysine is recommended to be 74% based on performance indices and carcass traits, and 77% based on bone traits.
Mohammad Hossein nemati; Hassan Almasi; Reza Masomi; mohamad hossein shahir
Volume 23, Issue 3 , September 2021, , Pages 447-457
Abstract
In this research the effect of using domestic (Multibehcil) and imported (Bioplus B2) probiotic supplements and Avilamycin antibiotics were investigated on performance, parameters of blood lipid and small intestine morphology by a sample of 360 male Ross-308 broilers aging 11 to 42 days-old in a completely ...
Read More
In this research the effect of using domestic (Multibehcil) and imported (Bioplus B2) probiotic supplements and Avilamycin antibiotics were investigated on performance, parameters of blood lipid and small intestine morphology by a sample of 360 male Ross-308 broilers aging 11 to 42 days-old in a completely randomized design with 6 treatments, 4 replications and 15 chickens per each replicate. Experimental treatments included 1) without additive (control), 2) diets containing 0.1% of commercial bioplus B2 probiotic, 3) diets containing 0.01% of avilamycin antibiotic and treatments 4) to 6) diets containing 0.05%, 1% and 0.15% of Multibehcil, respectively. The results showed that body weight and feed conversion ratio was improved by using bioplus B2 probiotic and antibiotic (P < 0.05). Body weight and feed conversion ratio in birds receiving 0.1% and 0.15% of Multibehcil were not significantly different from birds receiving bioplus probiotic. Feed intake was not affected by the experimental treatments. Humoral immune response and intestinal morphological parameters in birds receiving 0.15% of Multibehcil was not significantly different from the control group and it was statistically equal to the groups receiving bioplus probiotics and antibiotics. The LDL level of blood showed a significant decrease in Bioplus B2, antibiotic and 0.15% of Multibehcil treatments (P < 0.05). In general, the results indicated that using of Multibehcil at the level of 0.15 percent can be a suitable alternative for both Bioplus B2 and Avilamycin during grower and finisher period in broilers.
Hossien mohammadzadeh kratei; mohamad hossein shahir
Volume 22, Issue 3 , September 2020, , Pages 391-406
Abstract
The effects of increasing levels of L-leucine supplementation on performance, carcass traits, breast meat production and determination of digestible leucine requirement broilers during the grower period (15 to 30 days) using 240 Ross males broiler chickens in a completely randomized design with six dietary ...
Read More
The effects of increasing levels of L-leucine supplementation on performance, carcass traits, breast meat production and determination of digestible leucine requirement broilers during the grower period (15 to 30 days) using 240 Ross males broiler chickens in a completely randomized design with six dietary treatments (five repetitions and eight chicks per repetition) was investigated. Treatments include; a basal diet with 1.53% digestible leucine and five diets containing 1.63, 1.73, 1.83, 1.93, and 2.03% digestible leucine by adding synthetic L-leucine to the basal diet. By increasing the levels of digetible Leucin, body wieght gain, carcass and breast muscle percentage were increased, but feed conversion ratio and abominal fat percentage were decreasd (p < 0.05). The response trends to increasing levels of digestible leucine were quadratic for weight gain, feed conversion ratio, the relative weight of carcass, relative weight of breast muscle and relative weight of abdominal fat ( p < 0.05). Due to the better fit of the quadratic model to the response criteria, digestible Leucine requirements were estimated at 1.70, 1.72, 1.724, and 1.735% for body weight gain, feed conversion ratio, the relative weight of carcass and breast muscle; respectively. Based on the average of these estimates, the suggested digestible Leucine requirements of broilers in the grower period is 1.72%.
Somayeh Zamani afshar; Taher harkinezhad; abas Bahari; mohamad hossein shahir
Volume 20, Issue 2 , August 2018, , Pages 213-224
Abstract
Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a ...
Read More
Selection of live animals with minimum carcass fat in animal breeding programs will lead to increase in meat production at national level. The ligands of Wnt genes are of the effective factors in adipocyte cell differentiation. This study was aimed to assess the association between polymorphisms in Wnt10a and Wnt10b genes and carcass traits in 96 Afshari – Booroola Merino male lambs. In this study, DNA was extracted from blood samples using phenol-chloroform extraction method and polymerase chain reaction was performed for amplification a 663 bp fragment of exon III and a part of the second intron of Wnt10a gene and a 512 bp fragment of exon III Wnt10b gene. The results showed a polymorphism and three monomorphisms (a different allele compared to reference sequence of the gene) in the Wnt10a gene but all the sequences of the Wnt10b fragment were the same in studied region of the gene. Results of the sequencing lead to identification of four single nucleotide changes in Wnt10a gene in the studied area when compared to the reference sequence. One of the monomorphisms was in the intron and among other three nucleotide changes identified in exon III one was a missense in codon 139. After digestion with restriction enzymes HpaII at this codon, it was observed that all sampled lambs had mutant homozygous genotype compared to the reference sequence. This site was also evaluated in a number of Afshari (fat- tailed) and Zell (with the least fat tail) but again the same results were observed. Therefore, it seems that in this position the G is the wild type allele in the studied population.
Mohammad Hossein Nemati; Mohammad Hossein Shahir; Mohammad Taher Harakinezhad; Houshang Lotfollahian
Volume 15, Issue 1 , July 2014, , Pages 45-53
Abstract
This experiment was conducted to determine the effect of vitamin C (VC) and coenzyme Q10 (CoQ10) onimmunity response of broilers in cold stress condition using 500 male chicks in a completely randomizeddesign with 5 treatments and five replicates. Treatments were: positive control (PC, normal conditionsgrowth ...
Read More
This experiment was conducted to determine the effect of vitamin C (VC) and coenzyme Q10 (CoQ10) onimmunity response of broilers in cold stress condition using 500 male chicks in a completely randomizeddesign with 5 treatments and five replicates. Treatments were: positive control (PC, normal conditionsgrowth without antioxidants), negative control (NC, cold stress without antioxidants), cold stress + VC(300 mg/kg diet), cold stress + CoQ10 (40 mg/kg diet) and cold stress + VC + CoQ10 at above mentioneddoses. To induce cold stress, from day 15 until end, temperature fixed at 15oC. Vaccine titers, humeraland cellular immune response and relative immune organs weight were studied. Results showed that coldstress decreased relative spleen weight but there was no significant difference between treatments.Relative weight of bursa was increased in cold stress condition (P<0.01) and using antioxidant especiallyCoQ10 decreased it (P<0.05). Lymphocyte percent decreased in cold stress (P<0.05). Improved cellmediatedimmune response to subcutaneous injection of phytohemagglutinin and proliferation of Tlymphocyte in vitro were observed (P<0.05). In conclusion, use of antioxidants VC and CoQ10 in the coldstress condition improved immune system activity and decreased mortality
Mohammad Hosein Shahir; Afshin Heidariniya; Hamidreza Taheri; Abdollah Hoseini
Volume 15, Issue 1 , July 2014, , Pages 55-64
Abstract
In order to determine the appropriate standardized ileal digestible (SID) lysine requirement in growingmale turkey poults (28-49 day of olds), one hundred sixty birds were used in a completely randomizeddesign with eight treatments (8 levels of SID lysine: 1.15 (basal diet), 1.225, 1.3, 1.375, 1.45, ...
Read More
In order to determine the appropriate standardized ileal digestible (SID) lysine requirement in growingmale turkey poults (28-49 day of olds), one hundred sixty birds were used in a completely randomizeddesign with eight treatments (8 levels of SID lysine: 1.15 (basal diet), 1.225, 1.3, 1.375, 1.45, 1.525, 1.6,1.675 percent) in 4 replicates of 5 birds. Increasing of SID lysine levels increased body weight gain anddecreased feed conversion ratio. Dietary treatment of 1.6 percent SID lysine had the highest body weightgain and the lowest FCR compared to the basal diet (P<0.05). By using of broken line-linear ascending,quadratic polynomial and broken line- quadratic ascending models, SID lysine requirements for weightgain were 1.47±0.11, 1.57±0.04 and 1.49±0.05 and for FCR were 1.57±0.17, 1.61±0.02 and 1.59±0.08,respectively. In conclusion, SID lysine requirements for body weight gain ranged between 1.47 to 1.57and for FCR between 1.57 to 1.61 percent.